The question in this video is
Are you real?
What does this question really mean? Is the guy asking whether the apparent female (I don't know if she is a cyborg or not because I did not yet watch the TV series) is a human? So, is "real" a synonym for "human"? If that's the case, then the first implication (in the form of a question) of the statement
If you can't tell, does it matter?
in relation to AI is
- Can we create an AGI that is sufficiently similar to a human that we can't tell whether it's a human or not (by just normally interacting with it)?
Of course, it's not clear what we mean by "normally interacting". As far as I remember, this issue is also raised in the film A.I. Artificial Intelligence, where the AI (the kid interpreted by Haley Joel Osment) looks sufficiently real to the other kid, so he behaves as if he was a human kid, but then the human kid understands that he's a machine, and starts to behave differently (I hope I'm remembering the film correctly).
So, the second question that we could ask is
- Once we understand that it's not a human (for example, because it's made of other substances), would we humans start to behave differently and start treating the AGI differently?
As opposed to the first question, which is still an open problem, this second question can probably be answered by looking at our relationships with other humans (or entities, such as other animals). Often, we have an idea of a person. Once we discover something new about that person, which maybe we dislike, we may start to treat that person differently. I think this would very likely also happen in our eventual relationship with a sufficiently advanced AGI too, as depicted in the mentioned film.
Now, let me try to address the other question
What are the implications of this statement in relation to experience, in relation to the self?
I think that you're asking whether a sufficiently advanced AGI could be considered conscious or not. Of course, this is a very hard question to answer, because we still don't have a clear definition of consciousness or we don't yet agree on a standard definition, so I don't really have a definitive answer to this question. However, if consciousness is just a byproduct of perception and the ability to understand the world and its (physical) rules, then an AGI could be conscious (in a similar way that humans are also conscious). However, consciousness may not actually be necessary to correctly act in the world. In any case, the AI probably needs to know that it has a body and that it needs to protect it for its survival, if that's its main goal.